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human surfactant protein d rspd  (R&D Systems)


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    R&D Systems human surfactant protein d rspd
    <t>Surfactant</t> protein D (SPD) is synthesized by the gallbladder and promotes colonization of both the cecum and colon with Escherichia coli . A , SPD +/+ gut organs were harvested at baseline or post-cecal ligation and puncture (CLP) with Western blots performed for SPD or β -actin (loading control). Blots represent pooled samples, n = 2/group. Representative gel shown from three experiments. Lungs from SPD −/− and SPD +/+ mice were used as negative and positive controls, respectively. B , Gallbladder was isolated from SPD +/+ mice after CLP or sham surgery and from SPD −/− mice after CLP with Western blots performed for SPD or glyceraldehyde 3-phosphate dehydrogenase antibody (GAPDH; loading control). Blots represent pooled samples, n = 5–7/group. Lungs from SPD −/− mice and SPD +/+ were used as negative and positive controls, respectively. C , SPD −/− mice ( n = 9) were gavaged with recombinant surfactant protein D <t>(rSPD),</t> followed by gavage with green fluorescent protein (GFP)-labeled E. coli , and compared with SPD −/− mice gavaged only with GFP-labeled E. coli ( n = 9). After 24 hr, cecum and colon were harvested. GFP-labeled E. coli were then detected by culture (Mann-Whitney * p < 0.05, ** p < 0.01). CFU = colony forming units.
    Human Surfactant Protein D Rspd, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+surfactant+protein+d+rspd/Recombinant+Human+SP-D+Protein%2C+CF/pmc09119639-47-29-36
    Average 94 stars, based on 13 article reviews
    human surfactant protein d rspd - by Bioz Stars, 2026-08
    94/100 stars

    Images

    1) Product Images from "Surfactant Protein D Influences Mortality During Abdominal Sepsis by Facilitating Escherichia coli Colonization in the Gut"

    Article Title: Surfactant Protein D Influences Mortality During Abdominal Sepsis by Facilitating Escherichia coli Colonization in the Gut

    Journal: Critical Care Explorations

    doi: 10.1097/CCE.0000000000000699

    Surfactant protein D (SPD) is synthesized by the gallbladder and promotes colonization of both the cecum and colon with Escherichia coli . A , SPD +/+ gut organs were harvested at baseline or post-cecal ligation and puncture (CLP) with Western blots performed for SPD or β -actin (loading control). Blots represent pooled samples, n = 2/group. Representative gel shown from three experiments. Lungs from SPD −/− and SPD +/+ mice were used as negative and positive controls, respectively. B , Gallbladder was isolated from SPD +/+ mice after CLP or sham surgery and from SPD −/− mice after CLP with Western blots performed for SPD or glyceraldehyde 3-phosphate dehydrogenase antibody (GAPDH; loading control). Blots represent pooled samples, n = 5–7/group. Lungs from SPD −/− mice and SPD +/+ were used as negative and positive controls, respectively. C , SPD −/− mice ( n = 9) were gavaged with recombinant surfactant protein D (rSPD), followed by gavage with green fluorescent protein (GFP)-labeled E. coli , and compared with SPD −/− mice gavaged only with GFP-labeled E. coli ( n = 9). After 24 hr, cecum and colon were harvested. GFP-labeled E. coli were then detected by culture (Mann-Whitney * p < 0.05, ** p < 0.01). CFU = colony forming units.
    Figure Legend Snippet: Surfactant protein D (SPD) is synthesized by the gallbladder and promotes colonization of both the cecum and colon with Escherichia coli . A , SPD +/+ gut organs were harvested at baseline or post-cecal ligation and puncture (CLP) with Western blots performed for SPD or β -actin (loading control). Blots represent pooled samples, n = 2/group. Representative gel shown from three experiments. Lungs from SPD −/− and SPD +/+ mice were used as negative and positive controls, respectively. B , Gallbladder was isolated from SPD +/+ mice after CLP or sham surgery and from SPD −/− mice after CLP with Western blots performed for SPD or glyceraldehyde 3-phosphate dehydrogenase antibody (GAPDH; loading control). Blots represent pooled samples, n = 5–7/group. Lungs from SPD −/− mice and SPD +/+ were used as negative and positive controls, respectively. C , SPD −/− mice ( n = 9) were gavaged with recombinant surfactant protein D (rSPD), followed by gavage with green fluorescent protein (GFP)-labeled E. coli , and compared with SPD −/− mice gavaged only with GFP-labeled E. coli ( n = 9). After 24 hr, cecum and colon were harvested. GFP-labeled E. coli were then detected by culture (Mann-Whitney * p < 0.05, ** p < 0.01). CFU = colony forming units.

    Techniques Used: Synthesized, Ligation, Western Blot, Control, Isolation, Recombinant, Labeling, MANN-WHITNEY



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    human surfactant protein d rspd  (R&D Systems)
    94
    R&D Systems human surfactant protein d rspd
    <t>Surfactant</t> protein D (SPD) is synthesized by the gallbladder and promotes colonization of both the cecum and colon with Escherichia coli . A , SPD +/+ gut organs were harvested at baseline or post-cecal ligation and puncture (CLP) with Western blots performed for SPD or β -actin (loading control). Blots represent pooled samples, n = 2/group. Representative gel shown from three experiments. Lungs from SPD −/− and SPD +/+ mice were used as negative and positive controls, respectively. B , Gallbladder was isolated from SPD +/+ mice after CLP or sham surgery and from SPD −/− mice after CLP with Western blots performed for SPD or glyceraldehyde 3-phosphate dehydrogenase antibody (GAPDH; loading control). Blots represent pooled samples, n = 5–7/group. Lungs from SPD −/− mice and SPD +/+ were used as negative and positive controls, respectively. C , SPD −/− mice ( n = 9) were gavaged with recombinant surfactant protein D <t>(rSPD),</t> followed by gavage with green fluorescent protein (GFP)-labeled E. coli , and compared with SPD −/− mice gavaged only with GFP-labeled E. coli ( n = 9). After 24 hr, cecum and colon were harvested. GFP-labeled E. coli were then detected by culture (Mann-Whitney * p < 0.05, ** p < 0.01). CFU = colony forming units.
    Human Surfactant Protein D Rspd, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+surfactant+protein+d+rspd/Recombinant+Human+SP-D+Protein%2C+CF/pmc09119639-47-29-36
    Average 94 stars, based on 1 article reviews
    human surfactant protein d rspd - by Bioz Stars, 2026-08
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Surfactant protein D (SPD) is synthesized by the gallbladder and promotes colonization of both the cecum and colon with Escherichia coli . A , SPD +/+ gut organs were harvested at baseline or post-cecal ligation and puncture (CLP) with Western blots performed for SPD or β -actin (loading control). Blots represent pooled samples, n = 2/group. Representative gel shown from three experiments. Lungs from SPD −/− and SPD +/+ mice were used as negative and positive controls, respectively. B , Gallbladder was isolated from SPD +/+ mice after CLP or sham surgery and from SPD −/− mice after CLP with Western blots performed for SPD or glyceraldehyde 3-phosphate dehydrogenase antibody (GAPDH; loading control). Blots represent pooled samples, n = 5–7/group. Lungs from SPD −/− mice and SPD +/+ were used as negative and positive controls, respectively. C , SPD −/− mice ( n = 9) were gavaged with recombinant surfactant protein D (rSPD), followed by gavage with green fluorescent protein (GFP)-labeled E. coli , and compared with SPD −/− mice gavaged only with GFP-labeled E. coli ( n = 9). After 24 hr, cecum and colon were harvested. GFP-labeled E. coli were then detected by culture (Mann-Whitney * p < 0.05, ** p < 0.01). CFU = colony forming units.

    Journal: Critical Care Explorations

    Article Title: Surfactant Protein D Influences Mortality During Abdominal Sepsis by Facilitating Escherichia coli Colonization in the Gut

    doi: 10.1097/CCE.0000000000000699

    Figure Lengend Snippet: Surfactant protein D (SPD) is synthesized by the gallbladder and promotes colonization of both the cecum and colon with Escherichia coli . A , SPD +/+ gut organs were harvested at baseline or post-cecal ligation and puncture (CLP) with Western blots performed for SPD or β -actin (loading control). Blots represent pooled samples, n = 2/group. Representative gel shown from three experiments. Lungs from SPD −/− and SPD +/+ mice were used as negative and positive controls, respectively. B , Gallbladder was isolated from SPD +/+ mice after CLP or sham surgery and from SPD −/− mice after CLP with Western blots performed for SPD or glyceraldehyde 3-phosphate dehydrogenase antibody (GAPDH; loading control). Blots represent pooled samples, n = 5–7/group. Lungs from SPD −/− mice and SPD +/+ were used as negative and positive controls, respectively. C , SPD −/− mice ( n = 9) were gavaged with recombinant surfactant protein D (rSPD), followed by gavage with green fluorescent protein (GFP)-labeled E. coli , and compared with SPD −/− mice gavaged only with GFP-labeled E. coli ( n = 9). After 24 hr, cecum and colon were harvested. GFP-labeled E. coli were then detected by culture (Mann-Whitney * p < 0.05, ** p < 0.01). CFU = colony forming units.

    Article Snippet: SPD +/+ and SPD −/− mice were gavaged with 10 μL/g of ampicillin-resistant GFP-labeled E. coli (ATCC 25922GFP, 3.74 × 10 8 colony forming units/mL) with or without recombinant human surfactant protein D (rSPD) (Cat 1920-SP-050, R&D systems).

    Techniques: Synthesized, Ligation, Western Blot, Control, Isolation, Recombinant, Labeling, MANN-WHITNEY